CYTO U Upcoming Webinars
Collapse Designing Panels for the Study of Hematopoietic Stem Cells

Designing Panels for the Study of Hematopoietic Stem Cells

Date: 10/29/2020 12pm EST

                                          

Dr. Steffen Schmitt                                               Dr. Marcus Eich

German Cancer Research Center (DKFZ)            Hi-Stem gGmbH

 

About the Presenters

Steffen Schmitt studied biology at the Ruprecht-Karls-University Heidelberg and finished his phD with key aspects in immunology. Subsequent to a postdoc time, he went to the Johannes-Gutenberg-University in Mainz, where he built up the flow cytometric service at the Center for natural and medical sciences (NMFZ). Since 2007 Steffen Schmitt is heading the Flow Cytometry Core Facility of the German Cancer Research Center (DKFZ) in Heidelberg.

Marcus Eich studied biology at the Technical University Darmstadt and finished his PhD in 2011 in toxicology at the university medical center Mainz. After an additional post doc in Mainz he joined in 2015 the HI-STEM team and the Flow Cytometry Core Facility of the German Cancer Research Center (DKFZ) in Heidelberg

 

Webinar Description

The hematopoietic system is a very fascinating system to study as it can re-populate an entire system starting from just one cell. In this webinar we give an overview of the targeted subpopulations of the hematopoietic system and how we tried to combine different experimental tasks and setups in one panel. At the end we discuss some of the issues we came up with during the panel design and present a short outlook how to further improve the panel.

 

Learning Objectives

  • brief overview of the hematopoietic system
  • from a backbone panel to specialized subpopulations
  • combining different experimental setups in panel design
  • tips and tricks for this panel

Who Should Attend

PhD students, postdocs and technicans involved in hematopoietic stem cell research

Formats Available: Live Webcast + Streaming
Original Seminar Date: October 29, 2020

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Designing Panels for the Study of Hematopoietic Stem Cells
    Collapse Experimental Design and Quality Control for High-Dimensional Human Immunophenotyping Studies in Large Cohorts

    Experimental Design and Quality Control for High-Dimensional Human Immunophenotyping Studies in Large Cohorts

     

    Date: 10/22/2020 12pm EDT

     

    Presented by:

     

    Thomas Liechti

    Postdoctoral Researcher 

    ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health

     

    About the Presenter

    Thomas Liechti obtained his PhD in Immunology and Microbiology at the University of Zurich in 2017 and is currently a postdoctoral researcher in Mario Roederer’s group at the Vaccine Research Center of the National Institutes of Health in Bethesda (USA). His main interest is high-dimensional flow cytometry and human immunology. During his postdoctoral training, he established a 28-color flow cytometry sample processing and analysis pipeline to assess the contribution of genetic and environmental factors to human immune homeostasis 

     

    Webinar Summary

     

    High-dimensional flow cytometry enables the characterization of the human immune system at unprecedent depth and at a population-wide scale. However, flow cytometry panel design poses many caveats and requires careful testing of several reagents and panel iterations to find optimal combinations of reagents with the best possible performance, i.e. low spill-over spreading and high resolution. This webinar is the second part of a back-to-back webinar with Florian Mair. In the first part, Florian will cover the development and common caveats of high-parameter flow cytometry panels. In the second part of this webinar series, I will be talking about more specific caveats we encountered during the development of panels described in OMIP-051, -058 and -060.

    Large immunophenotyping studies including thousands of samples require several experiments over an extended period of time which is prone to inter-assay variation. Here, I will discuss strategies which we implemented to build an optimal staining and sample processing pipeline for an immunophenotyping study including >3000 samples and how we tracked and reduced technical (instrument performance) and experimental variation. In addition, I will give some outlook into data pre-processing including automated exclusion of data variation and compensation.

     

    Learning Objectives

    After attending these two back-to-back webinars, participants should have gained a solid base for tackling high-dimensional immunophenotyping assays on a variety of platforms, thus minimizing trial-and-error experiences and wasted experiments. Also the attendees will learn specific strategies for generating reproducible and high-quality data in large patient cohorts.

     

    Who Should Attend

    Anyone with an interest in efficient panel design: Immunologists, Scientists of any field doing polychromatic flowcytometry, SRL-users and SRL-leaders.

     

     

     

     

     

     

    Formats Available: Live Webcast + Streaming
    Original Seminar Date: October 22, 2020
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Experimental Design and Quality Control for High-Dimensional Human Immunophenotyping Studies in Large Cohorts
    Collapse Panel design - A Practical Guide for Successful Fluorescent Cytometry Panels from 10-40 Parameters

    Panel design – A Practical Guide for Successful Fluorescent Cytometry Panels from 10-40 Parameters

    Date: 10/21/2020 12pm EDT 

    Florian Mair

    Immunologist | Cytometry Specialist

    Fred Hutchinson Cancer Research Center

     

    About the Presenter

    Florian graduated with a PhD from the University of Zurich, Switzerland, in 2014 and is currently working at the Fred Hutchinson Cancer Research Center in Seattle, USA, as an immunologist. During the past decade, he has been involved extensively with different cytometry platforms (conventional, spectral and mass cytometry) as well as scRNA-seq techniques and developed an interest in applying novel analysis approaches for single cell data. He has been actively engaged in teaching flow cytometry courses, including systematic panel design and analysis of high-dimensional cytometry experiments. Florian is currently an ISAC Marylou scholar.

     

    Webinar Summary

     

    Over the past decade, technical improvements and new reagents have permitted fluorescent-based flow cytometry assays to measure up to 40 parameters. These complex assays require robust controls and thorough experimental planning, but there are currently few resources that provide a systematic approach for reliable panel design. Also, historical notions as to how fluorophores and controls should be chosen are sometimes at odds with the reality of modern panel design.

    In this webinar, we will provide a practical guide for successful fluorescent panel design for any complex panel from 10-40 (or more) parameters, both for conventional compensation-based as well as spectral cytometry.

    Specifically, we will cover the following topics:

    • Brief overview of signal detection in conventional and spectral flow cytometers
    • The concept and underlying cause of spreading error (SE)
    • How the spillover spreading matrix (SSM) can be efficiently used to guide panel design
    • Relevant relationships between SE, fluorophore brightness and antigen expression level
    • Step-by-step approaches towards building a new panel
    • An overview of essential controls and typical caveats

    This webinar is back-to-back with a webinar by Thomas Liechti (NIH, USA), who will be talking about how to best design and use complex panels for large study cohorts (100s-1000s of samples), including the use of appropriate controls and analysis approaches.

     

    Learning Objectives

    After attending these two back-to-back webinars, participants should have gained a solid base for tackling high-dimensional immunophenotyping assays on a variety of platforms, thus minimizing trial-and-error experiences and wasted experiments. Also the attendees will learn specific strategies for generating reproducible and high-quality data in large patient cohorts.

     

    Who Should Attend

    Anyone with an interest in efficient panel design: Immunologists, Scientists of any field doing polychromatic flowcytometry, SRL-users and SRL-leaders.

     

     

    Formats Available: Live Webcast + Streaming
    Original Seminar Date: October 21, 2020

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Panel design - A Practical Guide for Successful Fluorescent Cytometry Panels from 10-40 Parameters