CYTO U Upcoming Webinars
Collapse Cytometry Part A Spotlight: Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay

Cytometry Part A Spotlight

Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay

Thursday, June 7 at 10am Eastern (U.S. & Canada)

 

Presented by:

Tamara Lekishvili, Ph.D.
Researcher, Molecular and Cell Biology
LGC Group
Middlesex, U.K.

Moderated by Niga Nawroly

About the Presenter

Dr. Lekishvili received her Ph.D. from Tbilisi State University, Georgia (Biochemistry). Later she was awarded with the NATO scholarship and continued her research at NTNU (Norway) in neurobiology. After two dynamic post-doctoral positions at the University of Bath (Prion disease research group) and University of Birmingham (Breast Cancer research group), she joined LGC were she is a certified flow cytometry specialist within the cell and molecular biology team. Her major research focus is developing novel cell-based assays using different flow cytometry applications.

Webinar Summary

Standardisation of flow cytometry methods is required to support robust and reliable quality assessment of cells during bioprocessing. Fluorescent cell barcoding (FCB) overcomes some of the limitations of conventional flow cytometry methods, with improved accuracy and data quality. However, measurement variability across instruments or between analysts remains a challenge that must be addressed.

A modified fluorescent cell barcoding method using fixable viability dye eFlour 506 has been developed that reduces the potential for such variation. Proof of principle has been demonstrated for multiplexed immunophentoypic analysis using cultured human mesenchymal stromal cells (hMSCs) exposed to a variety of bioprocessing conditions during cell expansion.

This approach, which can easily be transferred to a variety of cell types, represents a further step towards achieving the rigour and robustness required for quality assessment of bioprocessing of cell therapy products, allowing more subtle variations in marker expression levels to be confidently observed.

See the original article in Cytometry Part A at: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23248

Learning Objectives

  • Describe the modified cytosolic fluorescent cell barcoding method
  • Provide the critical technical considerations during the FCB method development
  • Demonstrate the rapid hMSC immunophenotyping utilising FCB approach

Who Should Attend

Scientists and researchers interested in FCB by flow cytometry for the rapid and reproducible immunophenotyping.

 

 

Formats Available: Streaming, Live Webcast
Original Seminar Date: June 07, 2018
On-Demand Release Date: Available Now

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Cytometry Part A Spotlight: Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay
    Collapse Akadeum Microbubbles and Flow Cytometry - Technology, Tools, and What

    CYTO Innovation Presents:

    Akadeum Microbubbles and Flow Cytometry - Technology, Tools, and What's to Come

     

    Thursday, June 21 at 12:00pm Eastern (U.S. & Canada)

     

    Presented by:

    John Younger
    Chief Technology Officer
    Akadeum Life Sciences, Inc.

     

    About the Presenter

    John formed Akadeum Life Sciences in 2014 with his co-founder, Brandon McNaughton, to bring microbubble-based tools to the life sciences.  Buoyancy is a powerful alternative to magnetic methods for cell separation and offers exciting new ways of working with samples in research, diagnostic, and therapeutic applications.  Prior to joining Akadeum, John enjoyed a 21-year career as a physician scientist at the University of Michigan.  While his clinical practice was in emergency medicine, his laboratory focused on the ways in which living cells interact – for good or ill – with engineered materials.

    Webinar Summary 

    I’ll present a short technical background about buoyant cell isolation, highlighting how microbubbles differ from magnetic particles.  Next, I’ll share some ways that microbubbles are improving the ways that users are preparing samples for flow cytometry and FACS. To wrap up, I’ll review current products available, give a preview about what cell separation products are coming.  Lastly, for upcoming products we’ll discuss how you and your team can have early access to kits.

    Learning Objectives

    • How can microbubbles improve your research and development
    • How can microbubbles replace or be combined with your current magnetic methods?
    • What new Akadeum products are coming in 2018 and 2019?

    Who Should Attend

    Researchers who want a simple way to isolate cells with no columns and no magnets.

     

     

    Formats Available: Streaming, Live Webcast
    Original Seminar Date: June 21, 2018

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Akadeum Microbubbles and Flow Cytometry - Technology, Tools, and What
    Collapse Engineering Synthetic Immunity to Cancer and Beyond

    Engineering Synthetic Immunity to Cancer and Beyond

     

    Wednesday, July 11 at 12pm Eastern (U.S. & Canada)

     

    Presented by:

    Michael Milone
    Associate Professor of Pathology and Laboratory Medicine
    Associate Director, Toxicology Laboratory
    University of Pennsylvania
    Philadelphia, Pennsylvania, USA

    Moderated by Jonni Moore

     

    More information about this upcoming webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: July 11, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Engineering Synthetic Immunity to Cancer and Beyond
    Collapse Validation, Part 1: Instrument Validation

    Instrument Validation

    Part 1 of the Validation Webinar Series

    Monday, July 16 at 12pm Eastern (U.S. & Canada)

    Presented by:

    Cherie Green
    Senior Scientific Manager
    Genentech
    San Francisco, CA

     

    More details on this upcoming webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: July 16, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Validation, Part 1: Instrument Validation
    Collapse Quantitative Strategies for Cell Sourcing in Musculoskeletal Tissue Engineering

    Quantitative Strategies for Cell Sourcing in Musculoskeletal Tissue Engineering

    Wednesday, August 1 at 12pm Eastern (U.S. & Canada)

    Presented by:

    George Muschler, MD
    Director, Regenerative Medicine Laboratory
    Cleveland Clinic
    Cleveland, Ohio, USA

    About the Presenter

    Dr. Muschler is a nationally-recognized Cleveland Clinic Orthopaedic Surgeon who has devoted his career to advancing patient care through basic and clinical research, and quality management.  As a clinician-scientist, his research laboratory focuses now on adult stem cell biology and tissue engineering, with particular interest in improving methods for harvest and processing of human stem cells and progenitors to enhance tissue regeneration and repair, design and use of quantitative assays of stem cells and progenitor cells relevant to tissue health and aging, and understanding and modulating stem and progenitor cell growth and remodeling kinetics in settings of disease or injury.  Dr. Muschler has authorized over ten patents, including Colonyze, a quantitative automated image analysis system for assay of stem cells and progenitors.

    Webinar Summary

    Cells are the core resource for biological products and biomanufacturing.  No new tissue is formed without cells. However, cell populations from different donors and tissues are highly variable with respect to the concentration, prevalence and the biological potential.  Managing this variation is a profound challenge in the design, fabrication and quality control of cell therapies.  This webinar will discuss the basic research and the development of a robotic, image-based cell picking technology to determine quantitative factors that may predict cell/colony quality.

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: August 01, 2018

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Quantitative Strategies for Cell Sourcing in Musculoskeletal Tissue Engineering
    Collapse Cytometry Part A Spotlight: 16-Color Panel to Measure Inhibitory Receptor Signatures from Multiple Human Immune Cell Subsets

    16-Color Panel to Measure Inhibitory Receptor Signatures from Multiple Human Immune Cell Subsets

    Part of the Cytometry Part A Spotlight Series

     

    Thursday, August 9 at 12pm Eastern (U.S. & Canada)

     

    Presented by:

    Anna Belkina, MD, Ph.D.
    Associate Director
    Flow Cytometry Core Facility
    Boston University School of Medicine
    Boston, MA, USA

     

    Moderated by Pratip Chattopadhyay

     

    More information on this webinar will be posted soon!

     

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: August 09, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Cytometry Part A Spotlight: 16-Color Panel to Measure Inhibitory Receptor Signatures from Multiple Human Immune Cell Subsets
    Collapse Validation, Part 2: Assay Validation

    Assay Validation

    Part 2 of the Validation Webinar Series

    Monday, August 13 at 12pm Eastern (U.S. & Canada)

    Presented by:

    Virginia Litwin
    Vice President, Immunology
    Caprion Biosciences
    Montreal, QC, Canada

     

    More details on this upcoming webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: August 13, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Validation, Part 2: Assay Validation
    Collapse Validation, Part 3: Just Do It! Tying It All Together

    Just Do It!  Tying It All Together

    Part 3 of the Validation Webinar Series

    Monday, September 10 at 12pm Eastern (U.S. & Canada)

    Presented by:

    Teri Oldaker
    Clinical Laboratory Scientist
    Mission Viejo, CA

     

    More details on this upcoming webinar will be posted soon!

     

    Formats Available: Streaming, Live Webcast + Streaming
    Original Seminar Date: September 10, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Validation, Part 3: Just Do It! Tying It All Together
    CYTO U Archived Webinars