Proliferation Monitoring by Flow Cytometry
The second module in Lesson 2 of the course series
Lesson 2: Monitoring Cell Cycle Progression
Module B: DNA Cell Cycle Data Analysis Strategies
This is the second of four modules of Lesson 2 of the Proliferation course. Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.
The course concludes with a discussion of controls to include in proper experimental design and explains how to analyzed synchronized cell populations as seen when cell cultures are drug treated.
Lesson 1: Basics of the Cell Cycle is currently available here.
Lesson 2A: Principles and Methods is currently available here.
Lesson 2C: Practical Considerations & Troubleshooting is currently available here.
Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.
Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA
Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.
Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how DNA flow cytometric data is analyzed.
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.
To view the course, select "Topics & Pricing Information" and then select "Add to Basket."
If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.
For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.
This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.