Cytometry Part A Spotlight
Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay
Thursday, June 7 at 10am Eastern (U.S. & Canada)
Tamara Lekishvili, Ph.D.
Researcher, Molecular and Cell Biology
Moderated by Niga Nawroly
About the Presenter
Dr. Lekishvili received her Ph.D. from Tbilisi State University, Georgia (Biochemistry). Later she was awarded with the NATO scholarship and continued her research at NTNU (Norway) in neurobiology. After two dynamic post-doctoral positions at the University of Bath (Prion disease research group) and University of Birmingham (Breast Cancer research group), she joined LGC were she is a certified flow cytometry specialist within the cell and molecular biology team. Her major research focus is developing novel cell-based assays using different flow cytometry applications.
Standardisation of flow cytometry methods is required to support robust and reliable quality assessment of cells during bioprocessing. Fluorescent cell barcoding (FCB) overcomes some of the limitations of conventional flow cytometry methods, with improved accuracy and data quality. However, measurement variability across instruments or between analysts remains a challenge that must be addressed.
A modified fluorescent cell barcoding method using fixable viability dye eFlour 506 has been developed that reduces the potential for such variation. Proof of principle has been demonstrated for multiplexed immunophentoypic analysis using cultured human mesenchymal stromal cells (hMSCs) exposed to a variety of bioprocessing conditions during cell expansion.
This approach, which can easily be transferred to a variety of cell types, represents a further step towards achieving the rigour and robustness required for quality assessment of bioprocessing of cell therapy products, allowing more subtle variations in marker expression levels to be confidently observed.
See the original article in Cytometry Part A at: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23248
- Describe the modified cytosolic fluorescent cell barcoding method
- Provide the critical technical considerations during the FCB method development
- Demonstrate the rapid hMSC immunophenotyping utilising FCB approach
Who Should Attend
Scientists and researchers interested in FCB by flow cytometry for the rapid and reproducible immunophenotyping.