Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion

Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion

 

May 1, 2019

 

Presented by:

 

Paul Tillberg, Ph.D.
Lab Head
HHMI/Janelia

 

Moderated by Stephen Lockett

 

About the Presenter

 

Paul Tillberg completed a B.S. in Electrical and Materials Engineering at UC Berkeley before going on to complete his Ph.D. in Electrical Engineering at MIT, where he worked on technology development for the biological sciences. In the Boyden group, he conceived, designed, and co-led the development of Expansion Microscopy. He is currently a Fellow/Lab Head at the HHMI Janelia Research Campus, where he continues to develop and disseminate the expansion method in addition to other avenues of technology development for biology.

 

Webinar Summary

The Expansion Microscopy method improves the effective resolution of any optical microscope by ~4-fold by uniformly expanding biological specimens. Expansion is achieved by embedding the tissue in an ultra-swellable gel, followed by a few simple processing steps. The method is easy to adopt and well-suited as a histology core facility offering, as it is compatible with existing antibody and fluorescent protein labelling protocols without modification.

 

Learning Objectives

  • Discuss how to do expansion microscopy
  • Describe the variants associated with expansion microscopy
  • Learn the strengths and drawbacks to evaluate expansion microscopy for user specific applications

 

Who Should Attend

Any biologist interested in probing the nanoscale structure of fixed tissue. Basic histology and imaging skills are useful for the smooth adoption of Expansion Microscopy.

 

 

 

Seminar Information
Date Presented:
May 01, 2019 12:00 PM Eastern
Length:
1 hour
Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
Products
Streaming
ISAC Member Price: $0.00
Non-Member Price:$0.00