Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion

Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion

Wednesday, May 1st, 2019 at 12 pm EST (US & Canada)

Presented by

Paul Tillberg, PhD

Lab Head



Moderated by 

Stephen Lockett

Principal Scientist,
Director, Optical Microscopy and Analysis Laboratory

Frederick National Laboratory and Leidos Biomedical Research, Inc

About the Faculty

Paul Tillberg completed a B.S. in Electrical and Materials Engineering at UC Berkeley before going on to complete his Ph.D. in Electrical Engineering at MIT, where he worked on technology development for the biological sciences. In the Boyden group, he conceived, designed, and co-led the development of Expansion Microscopy. He is currently a Fellow/Lab Head at the HHMI Janelia Research Campus, where he continues to develop and disseminate the expansion method in addition to other avenues of technology development for biology.

Webinar Summary

The Expansion Microscopy method improves the effective resolution of any optical microscope by ~4-fold by uniformly expanding biological specimens. Expansion is achieved by embedding the tissue in an ultra-swellable gel, followed by a few simple processing steps. The method is easy to adopt and well-suited as a histology core facility offering, as it is compatible with existing antibody and fluorescent protein labelling protocols without modification.

Learning Objectives

-Discuss how to do expansion microscopy

-Describe the variants associated with expansion microscopy

-Learn the strengths and drawbacks to evaluate expansion microscopy for users' specific applications

Who Should Attend

Any biologist interested in probing the nanoscale structure of fixed tissue. Basic histology and imaging skills are useful for the smooth adoption of Expansion Microscopy.

Seminar Information
Date Presented:
May 01, 2019 12:00 PM Eastern
1 hour
Expansion Microscopy: Improving Resolution Through Uniform Specimen Expansion
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
ISAC Member Price: $0.00
Non-Member Price:$0.00