Fluorescence microscopes are an important tool for measuring cellular properties. The use of standard instrument performance criteria, such as limit of detection, linear dynamic range, and resolution (point spread function), and reference materials can allow minimal performance criteria to be established for an assay. By applying these minimal performance criteria, data generated by image cytometry instrumentation can assured to be comparable from day to day and from laboratory to laboratory. This tutorial will instruct students on methods available for establishing minimal instrument performance criteria for an image cytometry assay. Students will also be provided with a checklist that can be used to assure that fluorescence microscopy image intensity data is appropriate for quantitative analysis.
International Society for Advancement of Cytometry
American Society for Clinical Pathology
This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1.5 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.
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