Proliferation Monitoring by Flow Cytometry
Lesson 1: Basics of the Cell Cycle
Lesson 1 of the Proliferation course reviews the basic concepts of cell cycle biology, setting the stage for the more specific methods and applications discussed in Lessons 2 and 3. Topics discussed include:
- Phases of the cell cycle and molecular events associated with each
- Cell cycle checkpoints and regulators (cyclins and cyclin dependent kinases)
- The relationship between the cell cycle phase distribution of a sample and DNA content histogram
- The use of DNA content histograms in combination with metabolic labeling or cellular synchronization to determine kinetics of progression through the different cell cycle phases
Lesson 2A: Principles and Methods is currently available here.
Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.
Lesson 2C: Practical Considerations & Troubleshooting is currently available here.
Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand
Katharine A. (Kathy) Muirhead
SciGro, Inc., Midwest Office
Middleton, Wisconsin, USA
Paul K. Wallace
Department of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, New York, USA
To view the course, select "Topics & Pricing Information" and then select "Add to Basket."
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This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.
- To understand what cellular processes need to occur in order for a cell to enter the cell cycle and complete division.
- To know and understand the difference between cell cycle progression and cell division.
- To understand how flow cytometry is used to measure cell cycle parameters and division.