Proliferation Monitoring by Flow Cytometry

Lesson 1: Basics of the Cell Cycle

Lesson 1 of the Proliferation course reviews the basic concepts of cell cycle biology, setting the stage for the more specific methods and applications discussed in Lessons 2 and 3. Topics discussed include:

• Phases of the cell cycle and molecular events associated with each
• Cell cycle checkpoints and regulators (cyclins and cyclin dependent kinases)
• The relationship between the cell cycle phase distribution of a sample and DNA content histogram
• The use of DNA content histograms in combination with metabolic labeling or cellular synchronization to determine kinetics of progression through the different cell cycle phases

Lesson 2A: Principles and Methods is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

Authors:

Kylie M. Price

Hugh Green Cytometry Core Facility

Malaghan Institute of Medical Research

Wellington, New Zealand

Katharine A. (Kathy) Muirhead

SciGro, Inc., Midwest Office

Middleton, Wisconsin, USA

Paul K. Wallace

Department of Flow and Image Cytometry

Roswell Park Cancer Institute

Buffalo, New York, USA

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This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.