Proliferation Monitoring by Flow Cytometry

Lesson 1: Basics of the Cell Cycle

Lesson 1 of the Proliferation course reviews the basic concepts of cell cycle biology, setting the stage for the more specific methods and applications discussed in Lessons 2 and 3. Topics discussed include:

• Phases of the cell cycle and molecular events associated with each
• Cell cycle checkpoints and regulators (cyclins and cyclin dependent kinases)
• The relationship between the cell cycle phase distribution of a sample and DNA content histogram
• The use of DNA content histograms in combination with metabolic labeling or cellular synchronization to determine kinetics of progression through the different cell cycle phases

Lesson 2A: Principles and Methods is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

Authors

Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.

Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA

  - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.

Learning Objectives

• To understand what cellular processes need to occur in order for a cell to enter the cell cycle and complete division. 
• To know and understand the difference between cell cycle progression and cell division 
• To understand how flow cytometry is used to measure cell cycle parameters and division

CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.

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To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.