In recent years, technical advances have made it possible to obtain transcriptomic data (i.e., mRNA expression) from single cells.  This presentation will be focused on using this technology for studies of T-cells and will discuss:  a) stimulation of cells; b) purification and sorting of single cells; c) considerations for selecting mRNA targets and qualifying assay primers/probes; d) procedures and workflow for performing the assay; and e) data analysis considerations.  We will also discuss the power and limitations of transcription-based approaches to cytometry.

Course Details/Objectives:

Overview of why transcriptomics might be valuable in cytometry.  Methods for stimulation of cells, purification/sorting of cells, QC of assay and performing pre- and on-chip amplifications.  Data Analysis.  Summary/Conclusions.