Strategic and tactical decisions must be made at multiple points on the journey from the birth of an idea to commercialization. The selection of the type of investors from the different categories of investors, can provide insight into decision-making, including not only the usual stage distinction but also the investors' experience and knowledge affecting investor opinions on the company and the management team. The expectations of investors in entrepreneurial life sciences companies, from initial business plan formulation and presentation to post-investment management and board issues, can be daunting. The strategy of learning from customers over the lifetime of the company, is critical from first product launch to market prominence. Creating a global commercialization strategy that enables a company to "look" big while working as a lean startup. Continuing innovation around the core technology, maintaining close relationships iwth customers, and thinking and acting globally can lead a company from starup to market prominence. Instights and challenges will be shared from experiences on various companies such as AmCell, Quantum Dot, Guava, ForteBio and IntelliCyt.