In this tutorial, a step-by-step explanation of how t-SNE works will be presented. The tutorial will present some of the problems with the original implementation which dramatically limited its potential resolution of cytometry-defined cellular populations. A new variant of the method called Verity Cen-se’ will be presented that is considerably faster than the original method and presents populations in unprecedented high-resolution. Several examples and experiments demonstrating the value of this tool will also be presented.