Proliferation Monitoring by Flow Cytometry
The first module in Lesson 2 of the course series
Lesson 2: Monitoring Cell Cycle Progression
Module A: Principles and Methods
This is the first of four modules of Lesson 2 of the Proliferation course. Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess:
- How cells within a population of interest are distributed among the different cell cycle phases; and
- The kinetics with which cells of interest are progressing through the cell cycle
Lesson 1: Basics of the Cell Cycle is currently available here.
Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.
Lesson 2C: Practical Considerations & Troubleshooting is currently available here.
Authors
Kylie M. Price - Hugh Green Cytometry Core Facility, Malaghan Institute of Medical Research, Wellington, New Zealand.
Katharine A. (Kathy) Muirhead - SciGro, Inc., Midwest Office, Middleton, Wisconsin, USA
Paul K. Wallace - Department of Flow and Image Cytometry, Roswell Park Cancer Institute, Buffalo, New York, USA.
Target Audience
Individuals with a basic understanding of flow cytometry who are unfamiliar with, or wish to review, how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle.
CYTO University
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.