CYTO U Courses
Collapse Detecting Apoptosis by Flow Cytometry, Module 2: Caspase Activation

Detecting Apoptosis by Flow Cytometry

Module 2: Caspase Activation

This is the second course in the Apoptosis series on CYTO U. This course will review the phenomenon of apoptosis including its role in maintaining homeostasis of the immune system.  It will describe the morphological and biochemical changes that occur during apoptosis, and which form the basis for flow cytometric assays for this phenomenon.  It will then discuss the many assays for cell death.  The course will then cover how to design cell death assays that can measure multiple apoptosis characteristics simultaneously, how to analyze them on a flow cytometer, and how to interpret the data.  Finally, it will provide guidance for choosing the best apoptosis assay for particular cell types and experimental systems.

Objectives

After completion of this course, learners will be able to:

  • Understand the definition of apoptosis and its role in maintaining immune system homeostasis.
  • Understand the morphological and biochemical changes that occur in cell during apoptosis.
  • Understanding the laboratory assays used for the detection of apoptosis by flow cytometry.
  • Design multicomponent assays for apoptosis, analyze them on a flow cytometer, and correctly interpret the data.
  • Choose the correct apoptosis assays for different cell systems and experimental situations.

Authors


Bill Telford
Senior Associate Scientist
National Cancer Institute
National Institutes of Health

 


Jolene Bradford
Associate Director, Flow Cytometry Systems
Thermo Fisher Scientific

 


Karen Tamul
Instrument Field Application Scientist
EMD Millipore

 

Formats Available: Detecting Apoptosis by Flow Cytometry - Module 2: Caspase Activation
Original Seminar Date: June 17, 2020
On-Demand Release Date: Available Now

Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Detecting Apoptosis by Flow Cytometry, Module 2: Caspase Activation
    Collapse Detecting Apoptosis by Flow Cytometry, Module 1:  Background and Basic Techniques

    Detecting Apoptosis by Flow Cytometry


    Module 1: Background and Basic Techniques


    This series of lectures introduces investigators to the concept of apoptosis, presents basic techniques, and then explores more advanced methodologies.  The content emphasizes the building of multiparametric assays for measuring many aspects of apoptosis simultaneously, allowing the investigator to construct powerful assays for measuring cell death.


    Authors


    Bill Telford
    Senior Associate Scientist
    National Cancer Institute
    National Institutes of Health

     


    Jolene Bradford
    Associate Director, Flow Cytometry Systems
    Thermo Fisher Scientific

     


    Karen Tamul
    Instrument Field Application Scientist
    EMD Millipore

     

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    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."


    If you are not logged in to the CYTO U website, you will be directed to the ISAC website asking for your login information.


    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.


    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

    Formats Available: Detecting Apoptosis by Flow Cytometry - Module 1: Background and Basic Techniques
    Original Seminar Date: October 23, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Detecting Apoptosis by Flow Cytometry, Module 1:  Background and Basic Techniques
    Collapse Proliferation Monitoring, Lesson 2D:  Applications

    Proliferation Monitoring by Flow Cytometry

     

    The fourth and final module in Lesson 2 of the course series.

     

    Lesson 2: Monitoring Cell Cycle Progression

    Module D: Applications

     

    This is the final module in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2D “Applications" looks at how investigators have used the principles and methods discussed in Lessons 2A – 2C to monitor cell cycle status and cell cycle progression in their research fields of interest. Topics covered include tumor diagnosis and prognosis, anti-tumor mechanisms of therapeutic agents, and regulation of growth vs. quiescence or senescence in normal and neoplastic cells. 

     

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 1: Basics of the Cell Cycle is currently available here.

     

    Authors:

     

    Kylie M. Price

    Hugh Green Cytometry Core Facility

    Malaghan Institute of Medical Research

    Wellington, New Zealand

     

    Katharine A. (Kathy) Muirhead

    SciGro, Inc., Midwest Office

    Middleton, Wisconsin, USA

     

    Paul K. Wallace

    Department of Flow and Image Cytometry

    Roswell Park Cancer Institute

    Buffalo, New York, USA

     

    -------

     

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

     

    If you are not logged in to the CYTO U website, you will be directed to the ISAC website to enter your login information.

     

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

    Formats Available: Proliferation Monitoring, Lesson 2D: Applications
    Original Seminar Date: May 11, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2D:  Applications
    Collapse Proliferation Monitoring, Lesson 2C:  Practical Considerations & Troubleshooting

    Proliferation Monitoring by Flow Cytometry

     

    The third module in Lesson 2 of the course series.

     

    Lesson 2: Monitoring Cell Cycle Progression

    Module C: Practical Considerations & Troubleshooting

     

    This is the third of four modules in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument set-up, and data collection impact the reliability and quality of the resulting DNA histograms. Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.

     

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 1: Basics of the Cell Cycle is currently available here.

     

    Authors:

     


    Kylie M. Price
    Hugh Green Cytometry Core Facility
    Malaghan Institute of Medical Research
    Wellington, New Zealand

     


    Katharine A. (Kathy) Muirhead
    SciGro, Inc., Midwest Office
    Middleton, Wisconsin, USA

     


    Paul K. Wallace
    Department of Flow and Image Cytometry
    Roswell Park Cancer Institute
    Buffalo, New York, USA

     

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

     

    If you are not logged in to the CYTO U website, you will be directed to the ISAC website to enter your login information.

     

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

    Formats Available: Proliferation Monitoring, Lesson 2C: Practical Considerations & Troubleshooting
    Original Seminar Date: March 17, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2C:  Practical Considerations & Troubleshooting
    Collapse Proliferation Monitoring, Lesson 2B: Data Analysis Strategies

    Proliferation Monitoring by Flow Cytometry


    The second module in Lesson 2 of the course series


    Lesson 2: Monitoring Cell Cycle Progression

    Module B: DNA Cell Cycle Data Analysis Strategies


    This is the second of four modules of Lesson 2 of the Proliferation course.  Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.


    The course concludes with a discussion of controls to include in proper experimental design and explains how to analyzed synchronized cell populations as seen when cell cultures are drug treated. Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wishes to review) how DNA flow cytometric data is analyzed is encouraged to attend.


    Lesson 1: Basics of the Cell Cycle is currently available here.

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.


    Authors:


    Kylie M. Price
    Hugh Green Cytometry Core Facility
    Malaghan Institute of Medical Research
    Wellington, New Zealand


    Katharine A. (Kathy) Muirhead
    SciGro, Inc., Midwest Office
    Middleton, Wisconsin, USA


    Paul K. Wallace
    Department of Flow and Image Cytometry
    Roswell Park Cancer Institute
    Buffalo, New York, USA


    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."


    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.


    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.


    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.



    Formats Available: On-Demand
    Original Seminar Date: January 31, 2018
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2B: Data Analysis Strategies
    Collapse Proliferation Monitoring, Lesson 2A: Principles and Methods

    Proliferation Monitoring by Flow Cytometry

     

    The first module in Lesson 2 of the course series

     

    Lesson 2: Monitoring Cell Cycle Progression

    Module A: Principles and Methods

     

    This is the first of four modules of Lesson 2 of the Proliferation course.  Module 2A focuses on the principles and methods for the use of DNA binding dyes alone, or in combination with thymidine analogs, proliferation related antigens, and/or phase specific expression vectors, to assess: 

     

    1. How cells within a population of interest are distributed among the different cell cycle phases; and
    2. The kinetics with which cells of interest are progressing through the cell cycle

     

    Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wish to review) how flow cytometry is used to monitor the distribution or progression of cells through the cell cycle is encouraged to attend.

     

    Lesson 1: Basics of the Cell Cycle is currently available here.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

     

    Authors:

     

    Kylie M. Price

    Hugh Green Cytometry Core Facility

    Malaghan Institute of Medical Research

    Wellington, New Zealand

     

    Katharine A. (Kathy) Muirhead

    SciGro, Inc., Midwest Office

    Middleton, Wisconsin, USA

     

    Paul K. Wallace

    Department of Flow and Image Cytometry,

    Roswell Park Cancer Institute

    Buffalo, New York, USA

     

     

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

     

    If you are not logged in to the CYTO U website, you will be directed to the ISAC website to enter your login information.

     

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

     

     

     

    Formats Available: On-Demand
    Original Seminar Date: August 30, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 2A: Principles and Methods
    Collapse Image Cytometer Characterization and Calibration, Lesson 1:  Overview and Checklist for Benchmarking

    Image Cytometer Characterization and Calibration

    Lesson 1: Overview and Checklist for Benchmarking

    This course has been developed for core facility managers, clinical personnel involved in quality assurance and scientists interested in reproducibility of imaging measurements. 

    Author



    Research Scientist
    National Institute of Standards and Technology

    Learning Objectives

    Students should have a basic understanding of optical microscopy and hands-on experience with a fluorescence microscope.

     

    -----

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

     

    Formats Available: Online Course
    Original Seminar Date: April 10, 2017
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Image Cytometer Characterization and Calibration, Lesson 1:  Overview and Checklist for Benchmarking
    Collapse Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle

    Proliferation Monitoring by Flow Cytometry

     

    Lesson 1: Basics of the Cell Cycle

     

    Lesson 1 of the Proliferation course reviews the basic concepts of cell cycle biology, setting the stage for the more specific methods and applications discussed in Lessons 2 and 3. Topics discussed include:

    • Phases of the cell cycle and molecular events associated with each
    • Cell cycle checkpoints and regulators (cyclins and cyclin dependent kinases)
    • The relationship between the cell cycle phase distribution of a sample and DNA content histogram
    • The use of DNA content histograms in combination with metabolic labeling or cellular synchronization to determine kinetics of progression through the different cell cycle phases

     

    Lesson 2A: Principles and Methods is currently available here.

    Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

    Lesson 2C: Practical Considerations & Troubleshooting is currently available here.

     

    Authors:

     

    Kylie M. Price

    Hugh Green Cytometry Core Facility

    Malaghan Institute of Medical Research

    Wellington, New Zealand

     

    Katharine A. (Kathy) Muirhead

    SciGro, Inc., Midwest Office

    Middleton, Wisconsin, USA

     

    Paul K. Wallace

    Department of Flow and Image Cytometry

    Roswell Park Cancer Institute

    Buffalo, New York, USA

     

     

    -------

    To view the course, select "Topics & Pricing Information" and then select "Add to Basket."

     

    If you are not logged in to the CYTO U website, you will be directed to the ISAC website to enter your login information.

     

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can reset it on the ISAC Web site by clicking on the "Forgot Password?" link on the login page.

     

    This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

     

    Formats Available: On-Demand
    Original Seminar Date: September 02, 2015
    On-Demand Release Date: Available Now

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Proliferation Monitoring, Lesson 1: Basics of the Cell Cycle
    Collapse Flow Cytometry Biosafety

    Flow Cytometry Biosafety

    This course will provide a summary of biosafety principles as they apply to flow cytometry and cell sorting and provide an overview of applicable standards and risk assessment. Topics covered include:

    • Biosafety Principles and Procedures
    • Understanding Risk Assessment
    • Instrument-specific Standard Operating Procedures (SOPs)
    • Biosafety in Cell Sorting
    • Containment Testing

    Authors

    Kevin Holmes

    Steve Perfetto

    Hank Pletcher

    Ingrid Schmid

    Learning Objectives

    At the completion of this course, students will have a clearer understanding of the principles and practices of biosafety as it pertains to flow cytometry, in particular cell sorting. Additionally, you will have a list of resources to aid in risk assessment and the development of Standard Operating Procedures in your own lab. 

     

    To view the course, Click "Register Now."

    If you are not logged in to the CYTO U website, you will be directed to a new page asking for your login information.  

    For current ISAC members, your user name and password to CYTO U are the same user name and password you have for the members-only section of the ISAC Web site. If you cannot remember your password, you can re-set it on the ISAC Web site by clicking on the "Forgot Password?" link on the log-in page.

     

    Formats Available: On-Demand

    Approved Credit:
  • ASCP: 1 hour CMLE

  • Topics & Pricing InformationTopics & Pricing Information Flow Cytometry Biosafety