Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)

Presented by:

Kedar Narayan
Center for Molecular Microscopy
National Institutes of Health
Frederick, MD


About the Presenter

Kedar Narayan is the group leader for cellular imaging at the Center for Molecular Microscopy (CMM), at the National Cancer Institute. He earned a Ph.D. in immunology, and has a research background in chemistry, pathology, and biophysics. Kedar's work focuses on the use of emerging technologies for subcellular imaging. At the CMM, he is responsible for FIB-SEM technology development and for driving collaborative projects that use FIB-SEM, electron tomography and correlative imaging approaches to explore cellular architecture and mechanisms.


Webinar Summary

Focused ion beams (FIBs), previously restricted to the materials sciences and semiconductor fields, are rapidly becoming powerful tools for ultrastructural imaging of biological samples. In focused ion beam scanning electron microscopy (FIB-SEM), subcellular architecture of resin-embedded cells and tissue can be investigated by cycles of iterative FIB milling or "slicing"¯ of the sample followed by SEM imaging. The FIB can also be used as a sculpting tool to create specific specimen shapes that can be analyzed further by transmission electron microscopy or by methods that probe chemical composition.

FIB-SEM imaging can also be correlated with light or other imaging modalities to provide complementary image information. While not a high-throughput technique, FIB-SEM fills an important "imaging gap"¯ in biology, allowing for 3D ultrastructural imaging of large samples at nanoscale resolutions. Here I provide a primer to the application of FIB-SEM in biology, including a guide to the practical aspects of using the technology, as well as selected examples of its contribution to the generation of new insights into subcellular architecture and mechanisms underlying host-pathogen interactions.


CYTO U Webinar Recordings

A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all. Recordings of previously presented live webinars are also available.


CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community.  In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics.  These are available at no cost to ISAC members and for a nominal charge for non-members.  Webinars are free for all.



Seminar Information
Date Presented:
December 18, 2017 12:00 PM Eastern
1 hour
Registration Fee:
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
Recording Only
ISAC Member Price: $0.00
Non-Member Price:$0.00