Proliferation Monitoring by Flow Cytometry
The second module in Lesson 2 of the course series
Lesson 2: Monitoring Cell Cycle Progression
Module B: DNA Cell Cycle Data Analysis Strategies
This is the second of four modules of Lesson 2 of the Proliferation course. Module 2B provides an in depth discussion of DNA cell cycle data analysis strategies. The underlying concepts behind the characteristic cell cycle distribution are described followed by a description with examples of simple and complex approaches to modeling normal and abnormal DNA fluorescence data.
The course concludes with a discussion of controls to include in proper experimental design and explains how to analyzed synchronized cell populations as seen when cell cultures are drug treated. Anyone with a basic understanding of flow cytometry who is unfamiliar with (or wishes to review) how DNA flow cytometric data is analyzed is encouraged to attend.
Lesson 1: Basics of the Cell Cycle is currently available here.
Lesson 2A: Principles and Methods is currently available here.
Lesson 2C: Practical Considerations & Troubleshooting is currently available here.
Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand
Katharine A. (Kathy) Muirhead
SciGro, Inc., Midwest Office
Middleton, Wisconsin, USA
Paul K. Wallace
Department of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, New York, USA
To view the course, select "Topics & Pricing Information" and then select "Add to Basket."
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This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.