Proliferation Monitoring by Flow Cytometry

The third module in Lesson 2 of the course series.

Lesson 2: Monitoring Cell Cycle Progression

Module C: Practical Considerations & Troubleshooting

This is the third of four modules in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument set-up, and data collection impact the reliability and quality of the resulting DNA histograms. Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.

Lesson 2A: Principles and Methods is currently available here.

Lesson 1: Basics of the Cell Cycle is currently available here.

Authors:

Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand

Katharine A. (Kathy) Muirhead
SciGro, Inc., Midwest Office
Middleton, Wisconsin, USA

Paul K. Wallace
Department of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, New York, USA

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This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.