Single Cell Analysis of Autofluorescence Lifetime Images

November 19, 2019

Presented by:

Alex Walsh, Ph.D. 
Assistant Professor, Biomedical Engineering
Texas A&M University

Moderated by Silas J. Leavesley, Ph.D.

About the Presenter

Dr. Walsh completed her Ph.D. at Vanderbilt University where she developed an autofluorescence lifetime-based assay for determining the optimal cancer treatment strategy for individual patients. As a post-doc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons.  Currently, Dr. Walsh is an Assistant Professor in the Biomedical Engineering Department at Texas A&M University.

Webinar Summary
Fluorescence lifetime imaging (FLIM) of the endogenous fluorophores, NADH and FAD, co-enzymes of metabolic reactions, provides a label-free method to quantify cellular metabolism.  This webinar will review multi-photon fluorescence lifetime imaging methods, single cell segmentation, and intra-population heterogeneity analysis.  Examples will be shown for drug response in breast cancer organoids and activation of T cells.

Learning Objectives

• Define fluorescence lifetime and time-correlated single photon counting imaging methods
• Interpret label-free FLIM images of NAD(P)H and FAD
• Discuss segmentation and single-cell analysis techniques

Who Should Attend
Anyone interested in label-free imaging, fluorescence lifetime imaging, or single-cell analysis.