Single Cell Analysis of Autofluorescence Lifetime Images

Single Cell Analysis of Autofluorescence Lifetime Images


November 19, 2019


Presented by:


Alex Walsh, Ph.D. 
Assistant Professor, Biomedical Engineering
Texas A&M University


Moderated by Silas J. Leavesley, Ph.D.


About the Presenter

Dr. Walsh completed her Ph.D. at Vanderbilt University where she developed an autofluorescence lifetime-based assay for determining the optimal cancer treatment strategy for individual patients. As a post-doc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons.  Currently, Dr. Walsh is an Assistant Professor in the Biomedical Engineering Department at Texas A&M University.


Webinar Summary
Fluorescence lifetime imaging (FLIM) of the endogenous fluorophores, NADH and FAD, co-enzymes of metabolic reactions, provides a label-free method to quantify cellular metabolism.  This webinar will review multi-photon fluorescence lifetime imaging methods, single cell segmentation, and intra-population heterogeneity analysis.  Examples will be shown for drug response in breast cancer organoids and activation of T cells.


Learning Objectives

  • Define fluorescence lifetime and time-correlated single photon counting imaging methods
  • Interpret label-free FLIM images of NAD(P)H and FAD
  • Discuss segmentation and single-cell analysis techniques


Who Should Attend
Anyone interested in label-free imaging, fluorescence lifetime imaging, or single-cell analysis. 

Seminar Information
Date Presented:
November 19, 2019 12:00 PM Eastern
1 hour
Registration Fee:
Single Cell Analysis of Autofluorescence Lifetime Images
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
On Demand Only
ISAC Member Price: $0.00
Non-Member Price:$0.00