High-Speed Cell Sorter Biosafety and Containment Testing

December 18, 2019

Presented by:

Geoffrey Lyon, M.P.H.  
Yale Flow Cytometry Facility Manager/BSL-3 Sort Operator
Yale University School of Medicine

About the Presenter

      • Yale University Flow Cytometry Facility, 2003-present
      • ISAC Biosafety Committee Member, 2015-present 
      • ABSA International Course Presenter; 2012,2014, 2019 
      • Yale BSL-3 Subcommittee Member 
      • BSL-3 Certified Researcher and Trainer 
      • Discovered cell sorter AMS filter failures

Webinar Summary

This webinar explores the various biosafety aspects of high-speed cell sorting.  The emphasis of this presentation is the evolution and development of aerosol containment testing, including the new ISAC standard that uses a novel impactor and microspheres to test containment.  This new assay can be utilized to test sorters housed inside or outside of a biosafety cabinet.

Learning Objectives

• Examine the hazards and risks associated with cell sorting along with a detailed explanation of the latest containment testing method after this webinar.   
• Describe how to implement the new testing method into existing safety protocols to ensure cells sorters have adequate aerosol containment.    

Who Should Attend

Anyone who uses high-speed cell sorters, sort operators, health and safety professionals, or anyone interested in biosafety.