Slide-Free Histology via MUSE: UV Surface Excitation Microscopy for Imaging Unsectioned Tissue by Richard Levenson

Richard Levenson, MD, FCAP, is Professor and Vice Chair for Strategic Technologies in the Department of Pathology and Laboratory Medicine, UC Davis. He trained in medicine at University of Michigan and pathology at Washington University, and is Board-certified in Anatomic Pathology. A faculty position at Duke was followed by an appointment at Carnegie Mellon University where he helped develop multispectral imaging approaches for pathology and biology. In 1999, he joined CRI to become VP of Research, and served as Principal Investigator on federally funded research to develop multispectral microscopy systems and software for molecular pathology and diagnostics, three-dimensional small-animal imaging, optical dynamic contrast techniques, and birefringence microscopy. He serves on NIH, NCI and NSF review panels, is associate editor of Analytical Cellular Pathology, section editor for Archives of Pathology, and is on the editorial boards of Laboratory Investigation, Cytometry Part A, and Oncopathology. 

Webinar Summary:

Slide-free methods for rapid tissue histological analysis can cut hours off usual pathology slide preparation procedures. One approach to accomplish this is MUSE (Microscopy with UV Surface Excitation), which exploits shallow penetration of UV light to excite fluorescent signals from only the most superficial tissue elements. The method is non-destructive, and eliminates the need for conventional histology processing, formalin fixation, paraffin embedding, or thin sectioning. It requires no lasers, confocal, multiphoton or optical coherence tomography optics. MUSE generates diagnostic-quality histological images from fresh or fixed, but unsectioned tissue that can resemble conventional hematoxylin- and eosin-staining, with enhanced topographical information, and can do this rapidly, simply and inexpensively.


This continuing medical laboratory education activity is recognized by the American Society for Clinical Pathology for 1 CMLE credit. ASCP CMLE credits are acceptable for the ASCP Board of Registry Certification Maintenance Program.

Seminar Information
Date Presented:
September 22, 2015 12:00 PM Eastern
1 hour
Slide-Free Histology via MUSE: UV Surface Excitation Microscopy for Imaging Unsectioned Tissue by Richard Levenson
Individual topic purchase: Selected
American Society for Clinical Pathology
CMLE: 1.00
ISAC Member Price: $0.00
Non-Member Price:$0.00